Background: There was a continuous argument regarding the impact of Vitamin D de?ciency in pregnancy and the adverse maternal and fetal outcomes. Some demonstrated harmful effects of hypovitaminosis D while other denied association of deficiency and fetomaternal outcome.
Aim of work: To assess the correlation of serum Vit D level and different pregnancy fetomaternal complications and assess whether feto-maternal morbidities are associated with hypovitaminosis D or not.
Patients and Methods: 322 participants who meted the inclusion criterias were classified into 2 groups. The first one was 110 pregnant with normal pregnancy regarding both fetal and maternal conditions were selected as control group. The second one was 212 pregnant suffering from pregnancy complications and were further subdivided into,58 cases with severe preeclampsia toxemia associated with intrauterine growth restriction, 82 case with GDM, 26 cases of abortion, 16 cases undisturbed ectopic pregnancy,14 case with premature rupture of membranes=PROM and 16 cases with inevitable preterm labor. Serum recovered from peripheral blood samples by centrifugation was aliquot stored until used at -80 oC. Specific quantitative ELISA immunoassay kits from Sunlong Biotech Co. Ltd. were used to measure 25-OH-VitD (in ng/mL) and 1, 25-DiOH-VitD (in pg/mL) (cat# SL2762Hu and SL2845Hu, Hangzhou, Zhejiang, China). The direct DiOH-VitD/OH-VitD ratio was calculated for each patient.
Results: 25 -OH -Vit D showed significant differences only between control and GDM groups while there were no significant differences among control and other groups. On the other hand, 1, 25-DiOH-VitD assay showed very marked significant reductions in comparing control versus all of 6 complicated groups. Very similar to 1, 25-DiOH-VitD pattern, DiOH-VitD/OH-VitD ratio showed no significant difference among the complicated groups. However, there were very marked significant reductions comparing control vs. all of the 6 complicated groups
Conclusions: There is a strong association of vit D status and certain pregnancy complications.1, 25 Di-OH-Vit D assay seems to be more reliable than 25-OH-Vit D and could be used as a suitable biomarker in assessment of vit D status in pregnancy. Vit D supplementation during pregnancy may assist in reducing related complications.
What will audience learn from your presentation?
• Knowing the nature, types ,sources of vit D
• Role and fubnction of vit D
• Requirement for vit D
• Relation of vit D to different pregnancy complications
• Definite impact of hypovitaminosis in pregnancy
• Innovative method to use 1,25 di OH vit D as a more relevant biomarker to asses D level