More than two decades ago, the field of bioinformatics arose as a means of advancing biological research. Bioinformatics is an interdisciplinary branch of research that includes biology, mathematics, and computer science. It has become a smoothing biological science equipment that saves as much information as possible. Every day, high throughput sequencing (HTS) technologies make massive amounts of biological data available to scientists. Such important discoveries are currently being evaluated using bioinformatics and algorithmic methodologies. Bioinformatics, on the other hand, can be used to extract, mine, interpret, and sort material with cross-linking references that are accessible to help understand biological intricacies in this vast natural library and provide fresh insight. Over the last few decades, genomics and bioinformatics have been crucial in gaining a better understanding of heritable abnormalities that cause human infertility. More recently, genome biological techniques have been used in reproductive biology to obtain insight into the molecular processes underlying normal and abnormal gametogenesis and gamete function.