Title : Peritoneum an organ and its role in reproductive regeneration
Abstract:
Historically peritoneum has been a neglected anatomical structure, however recent research identifies it as an important functional organ. Apart from lining and protecting the abdominal organ it plays a major role in tissue repair, immunological response and tissue regeneration. The mesothelial cells of the peritoneum plays an important role in its function as an organ. The mesothelial cells of the peritoneum are extremely important as an organ functional unit. The microvilli and the intercellular junctions help in immune signaling and tissue repair. These mesothelial cells have an active role in immune defense and inflammatory responses. The cytokines, growth factors, and extracellular matrix components secreted by the mesothelial cells help in tissue repair and immune activity.
The peritoneum has a remarkable ability to repair itself, following injury. This regenerative capacity has been documented. The laparoscopic incisions and dissection areas in perotoneum get covered in 24 hours. The most important function of the mesothelial cells is their ability to undergo metaplasia and changing into different structures. Its evident by the conditions like endometriosis, ovarian malignancies and many other pathological conditions.
The embryogenesis of mullerian ducts is by peritoneal invagination. Having the same parentage is the main hypothesis of our research in peritoneal vaginoplasty. This has been documented in more than 200 plus cases of vaginoplasty (publication). The next research is developing the uterine endometrial lining and developing the myometrial tissue.
The results of the animal experiments are presented herewith documenting peritoneal conversion into endometrial lining. The progenitor cells and the genomes activated during the metaplasia are also identified. The present study evaluated the effect of the treatment on the expression of key pluripotency-associated genes OCT-4, LEFTY, and NANOG in rabbit uterine tissue, using both quantitative real-time PCR and immunohistochemical analysis. The results consistently demonstrated a time-dependent and treatment-specific upregulation of these markers, indicating activation of stem-cell–related molecular pathways.
